Frequently Asked Questions

If you have a question, or require help with your yoghurt making, please call (07) 3808 2576 or email us with as much information about your question or problem as possible at

What is the shelf life of the yoghurt and probiotic cultures ?

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Room temperature   weeks
Refrigerator   months
Freezer   years

How should I store my yoghurt culture ?

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The yoghurt and probiotic cultures are freeze dried, and require "long term storage" in freezer. The cultures also readily absorb moisture out of the air so should be kept in airtight containers which should only be opened at room temperature.

We supply two sterile jars for storage of the cultures. Please see the next FAQ for more information regarding this.

Why do we send two Sterile Jars ?

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The two jar storage and dosing method helps keep the culture in the best condition, helping prevent cross contamination and helping with dose control. We recommend placing approximately 90% of the culture in one jar, rarely opened, and have another jar with a small amount of culture in, as your working supply.

When a cold item is taken from a freezer, moisture condenses onto it, out of the air. This is true of the opened sterile jar with culture inside.

With Yoghurt Culture and the Probiotic Culture both requiring storage in a freezer, and being so concentrated, the storage jar will be opened many times (if kept only in one jar), before being used up, and it is impossible to keep the culture 100% dry.

This also has a side benefit of reducing the chance of the contamination of the culture. If the working stock does get contaminated, at least the main supply is still OK.

Does the Yoghurt Culture contain any dairy ?

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Sacco's cheese and yoghurt cultures are grown on a dairy based medium. At the end of propagation, the starter culture cells are physically separated from this medium, then concentrated and freeze-dried. Very little, if any of the dairy component ends up in the final starter culture. However because people with allergies to dairy protein can be sensitive to parts per million (ppm) levels, Sacco cannot guarantee that such levels are not present in their cultures, and therefore declare that all of their cheese and yoghurt cultures may contain traces of dairy.

We do have a specialist Non-Dairy Yoghurt Culture, actually grown on a non dairy base, so as not to contain any dairy at all.

Does the Yoghurt Culture contain any allergens ?

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We have Allergen Declarations from Sacco, who supply our Yoghurt and Probiotic cultures.

This document is the dairy cultures allergen statement and here is the non-dairy allergen statement.

Why should I heat the milk to 90° C. ?

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Heating the milk to 90° C for a short time breaks down a protein in milk, enabling the culture to do its work better, making a thicker heartier yoghurt.

If using UHT milk, there is no need to do this as it has already been done as part of the "Ultra Heat Treatment", that UHT stands for. Just heat your milk to 40° C before adding the culture.

Do I have to add the extra milk powder ?

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Adding the extra milk powder simply gives the culture more lactose and dairy protein to "eat", this makes the yoghurt thicker and heartier without adding extra thickeners, such as agar-agar or gelatin.

If you wish to make a thinner "drinking yoghurt" simply leave out the milk powder.

If you wish to make a thicker yoghurt without using milk powder, hanging the yoghurt in a tight weave cheese cloth is a way of getting it to thicken by draining the whey. This will reduce the amount of end product.

How is Powdered Milk made, and does it have additives ?

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Powdered milk is made from fresh, pasteurised milk. First, the milk is concentrated in an evaporator until 50% of the milk solids remain. Next, the concentrated milk is sprayed into a heated chamber where the water almost instantly evaporates, leaving behind tiny dry milk particles.

Powdered milk has the following ingredients, that all came from the milk:

  • Nonfat Dry Milk
  • Vitamin A Palmitate
  • Vitamin D3

Instant powdered milk has a small amount of soy (322) as an emulsifier, this what prevents clumping and makes mixing 'instant'.

If your powdered milk has any of the following listed, it is not real powdered milk:

  • sweet dairy whey
  • non-fat dry milk solids
  • partially hydrogenated vegetable oil (contains one or more of the following: canola oil and/or soya oil)
  • corn syrup solids
  • sodium caseinate
  • dipotassium phosphate
  • propylene glycol monostearate
  • mono and diglycerides
  • carrageenan

All powdered milks we have seen in Aussie supermarkets are good quality products.

My yoghurt has a stringy consistency, how can I fix this ?

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A stringy, almost slimy consistency is typical of yoghurt fermented at too low a temperature.

With thermos style yoghurt makers try increasing the temperature, and also monitoring the temperature over the fermentation period to make sure that it stays with the temperature range of 37 to 43° C.

With electric yoghurt makers if the milk goes in too cold, the cultures may experience unbalanced growth as the milk slowly heats up to 40° C, this unbalanced culture growth may lead to an undesirable texture. We have found that preheating the milk to between 35 and 40° C, negates this effect.

Can I use Soy or other milks ?

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Yes! All of our yoghurt culture work with soy milk, almond milk, and coconut cream.

The addition of a small amount of sugar to act as food for the cultures may be needed depending on the sugar content of the milk, and a thickening agent is needed for almond and coconut yoghurts.

Do I still heat dairy milk alternatives to 90° C ?

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Yes and no. For soy milk and coconut cream simply bring the temperature up to 40° C, add some sugar if desired, a thickening agent if required, and add the culture. Then keep your inoculated soy milk warmed to around 40° for 12 to 24 hours, and when it has turned into a nice thick yoghurt refrigerate.

We have found that almond milk will sometimes 'split' into two layers during fermentation. This can be prevented or at least reduced by heat treating your almond milk to 90° C for ten minutes prior to cooling, and continuing with ther fermentation process.

How much culture do I use ?

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One of the most frequent questions I get asked by customers is how much culture do I use when making yoghurt.

Cultures come in packets that are labelled with how many litres of milk they will inoculate. Our yoghurt cultures come in a packet that will inoculate 100 litres of milk. How much is in the packet will vary from culture to culture and batch to batch of the same culture. Remember, it’s alive, so it will not be the same for every batch that is grown. Culture is not measured by weight or volume, but rather by its rate of activity.

The activity rate of a culture is most easily determined by measuring the acid production or decrease in pH of the milk it is added to. In order to have an easily understandable system the growing conditions are standardised by having the same medium, temperature and time. For example, the use of a standard weight/volume percentage concentration (w/v) reconstituted skimmed milk, at 30oC for six hours.

So if you get a packet of yoghurt culture that does 100 litres and you want to make two litres of yoghurt, you would use 2/100 of the packet (1/50th), regardless of whether the weight has changed from batch to batch for that culture.

Bring the sachet to room temperature before opening it for the first time. This should only take a few minutes and reduces the effect of condensation causing some of the culture to stick to the inside of the sachet. Cut all the way across the top of the sachet and using a sharp, pointed knife empty the culture into one of the sterile jar supplied. Using a shard, pointed knife allows you to get right into the corners of the packet to get all of the culture out.

Once you have all of the culture out of the packet into the first sterile jar, take a look at it. This is enough for 100 litres and is your main ‘storage jar’. Now estimate 1/10th of the contents of the first sterile jar and place into the sterile second jar. This becomes your ‘working supply’, label both jars and store in the freezer.

Your ‘working supply’, being approximately 1/10th of the original supply of culture, will make approximately 10 litres of yoghurt. Now you are getting the idea of how small the amount of culture is that you need to use.  Use the tip of a sharp, pointed knife to get the amount of culture you need to use for the size batch you are going to do. I usually do a two litre batch, but you might be doing a one litre batch.  

The most common problem people have with this is using too much culture. If you do accidently use not enough; no big problem. Remember the culture is alive and if kept at the correct temperature, it is going to double about every 30 minutes. The Green Living Australia yoghurt maker is designed to keep the temperature accurate over extended fermentation times, so wait half an hour longer and you have doubled your culture. 

From time to time I am asked why we do not supply a simpler way to do this. Why don’t we have a one sachet system so you can just tear open a packet and add it; no measuring; no guessing. That would be great, but unfortunately, so would the cost. There is a product out there like that and we are compared to them all the time. How easy would it be if the Green Living Cultures could be packed in these convenient packets? Well let’s have a look at what you are getting in regards to convenience and how much you are paying for it.

I did a Google search on yoghurt making cultures and I found the price to be $4.20 for a packet that makes one litre.  It is mostly powdered milk and you just add water. So for 100 litres that is $420.00. Now compare that to $17.95 for 100 litres of the Green Living Australia culture. You do have to add the Green Living Australia culture to milk, so you are up for another $100.00 there. Overall saving to you is $302.05. Is that worth a few minutes of your time? 


Can I make Yoghurt with just your culture, or do I have to use a probiotic too ?

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Yes you can use just the Yoghurt Culture to make yoghurt. The probiotic are used in conjunction to the culture for those people wishing for an end product that is Probiotic

Can I make Yoghurt with just your probiotic culture, without the yoghurt culture ?

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No. The texture and flavour cultures are in the yoghurt culture, but not in the probiotic cultures.

Can I clone more yoghurt from the yoghurt I make with your Yoghurt Culture ?

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Yes you can clone your next few batches of the yoghurt you make from the yoghurt you make with our culture, although when cloning from a previous batch, you are cloning all of the bacteria present in the yoghurt, including any contaminants. This is why cloned yoghurt typically becomes runnier, and does not set as well in the second and third generation. It may be considered a false economy, risking the disappointment of failed yoghurt, to save 17 cents worth of culture.

Why is there a special non-dairy culture, if both the Mild and Tangy Yoghurt Cultures work with Soy Milk ?

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The Mild and Tangy yoghurt cultures are grown on a dairy base and, while they are separated from the dairy base, there may be the odd molecule of dairy left behind. Some people are so allergic to dairy, this is enough to be a serious issue.

Other people such as Vegans just wish to avoid dairy altogether.

Why has my yoghurt culture started clumping together ?

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The clumping is caused by the culture absorbing moisture. The moisture can be absorbed directly from the air and also from moisture condensing out of the air onto the inside of the storage container if opened while cold. So we always allow the culture to come to room temperature and we keep the jar closed as much as possible to minimise the amount of fresh air coming into contact with the culture. I also try to avoid making yoghurt on a really damp or humid day unless I run the air conditioner for a while to dry the air out. I also don't spend any time trying to judge 1/10th perfectly accurate, I just know to use an extremely small amount, making the time the jar is open probably less than 20 seconds.

How do I test my yoghurt maker ?

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To find out what temperature your machine runs at and whether your machine experiences 'temperature creep' is very easy. Simply place a litre or so of tap water in the yoghurt canister at about 40 deg C. and then check the temperature periodically over the next 12 to 24 hours. Electric yoghurt makers should keep the water between 37 to 43 degrees and this temperature should be consistent even over longer fermentation times.

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